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🔬🧫🧬 The commensal bacterium Faecalibacterium prausnitzii has unique anti-inflammatory properties, at least some of which have been attributed to its production of MAM, the Microbial Anti-inflammatory Molecule.
👩⚕️ Recent taxonomic studies on Faecalibacterium strains have revealed the existence of various species. In this talk, I will address the question of whether MAMs from different species display distinct anti-inflammatory properties.
🧬 We first performed wide-scale identification, classification, and phylogenetic analysis of MAM-like proteins encoded in different genomes of Faecalibacteria. When combined with a gene context analysis, this approach distinguished at least 10 distinct clusters of MAMs, providing evidence for functional diversity within this protein.
👨🔬 We then selected 11 MAMs from various clusters and evaluated their anti-inflammatory capacities in vitro. A wide range of anti-inflammatory activity was detected. MAM from the M21/2 strain had the highest inhibitory effect (96% inhibition), while MAM from reference strain A2-165 demonstrated only 56% inhibition, and MAM from strain CNCM4541 was almost inactive. These results were confirmed in vivo in murine models of acute and chronic colitis.
🧫 These results were confirmed in vivo in murine models of acute and chronic colitis. This study provides insights into the family of MAM proteins and generates clues regarding the choice of F. prausnitzii strains as probiotics for use in targeting chronic inflammatory diseases.
🎤 To find out more, meet Jean-Marc Chatel from INRAE France at the 17th IPC2024 in Prague, 18-21 June 2024 in Czechia, see the entire programme at https://probiotic-conference.net/ and...
Hit the bell 🔔🔔🔔 to stay tuned on to International Scientific Conference on #Probiotics, #Prebiotics, #Gut #Microbiota and #Health - #IPC2024 and get updated on probiotic, prebiotic #science and #microbiota #research.
🔬🧫🧬 The gut microbiome is increasingly recognised for its role in Alzheimer's disease (AD). Recent studies have proposed an involvement of the gut microbiome in AD pathogenesis by influencing human metabolism via metabolic cross-talk with the host.
👩⚕️ However, mechanistic pathways connecting the gut microbiome to AD pathogenesis remain unknown. Recent developments in the fields of gut metagenomics and metabolic modelling have made it possible to generate microbiome personalised whole-body metabolic models (WBMs).
🧬 WBMs can be algorithmically interrogated to describe metabolic phenotypes of the host-gut system, by calculating metabolite production capacities. Due to the deterministic nature of WBMs, these metabolite production capacities can be linked to individual microbes in the WBMs, resulting in mechanistic hypotheses of host-microbiome cross-talk.
👨🔬 For this study, we generated WBMs from a large cross-sectional cohort with the aim to employ them for obtaining mechanistic hypotheses on the link between gut microbiome alterations and risk of developing AD.
🧫 Taken together, our findings suggest a potential sex-specific involvement of the gut microbiome in AD pathogenesis. Moreover, we hypothesise preclinical shifts in bile acid producing bacteria, such as Eggerthella lenta, to be linked to an increased risk of developing AD. If confirmed, antibiotic targeting of these gut microbes could provide a potential means of delaying the onset of AD.
🎤 To find out more, meet Tim Hensen from the University of Galway at the 17th IPC2024 in Prague, 18-21 June 2024 and...
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🔬🧫🧬 Lactiplantibacillus plantarum WCFS1® is one of the most independently studied probiotic strain. It has shown in vitro, in vivo and clinical potential for intestinal microbiome modulation, immunomodulation, anti-inflammatory effects, maintaining epithelial integrity, lowering triglycerides and LDL levels, increasing leptin level and lowering weight.
👩⚕️ The purpose of this prospective clinical study was to evaluate the impact of a formulation containing three probiotic strains (including WCFS1®) on the intestinal microbiome and on weight management.
🧬 A probiotic formula was developed with three probiotic strains (Biena Inc., Saint-Hyacinthe, QC, Canada) that have previously been evaluated for their high survival during the intestinal passage as well as their clinical efficacy. 19 human volunteers (10 females / 9 males) were selected based on their BMI ≥30 and their waist circumference (females ≥80 cm; males ≥104 cm). During their first visit, their height, weight and waist circumference were measured and they were given a Stool Collection Kit (CosmosID®) for at-home self-sampling of their intestinal microbiome (T0). DNA extraction, library preparation, 20M Total Illumina DNA Sequencing & Metagenomic Analysis were performed using the CosmosID® cloud-based platform to obtain strain-level identification and abundance.
👨🔬 Impact on weight management: Out of 19 volunteers, 14 have shown a decrease in weight and/or waist circumference, (including 9 out of 10 of the female subjects), with 6 showing a decrease of both parameters. However, the difference was not statistically significant (P > 0.05).
👨🔬 Impact on microbiome: All three probiotic species found in the formula were significantly enriched between T0 and T1 samples (B. lactis: P=0.001; L. plantarum: P=0.001; L. rhamnosus: P=0.027). Further analysis demonstrated that this enrichment was only significant for the female subjects
🧫 The formula was shown to
🔬🧫🧬 Bioengineered probiotics enable new opportunities to improve colorectal cancer (CRC) screening, prevention and treatment.
👩⚕️ Here, first, we demonstrate selective colonization of colorectal adenomas after oral delivery of probiotic E. coli Nissle 1917 (EcN) to a genetically-engineered murine model of CRC predisposition and orthotopic models of CRC.
🧬 We next undertake an interventional, double-blind, dual-centre, prospective clinical trial, in which CRC patients take either placebo or EcN for two weeks prior to resection of neoplastic and adjacent normal colorectal tissue (ACTRN12619000210178).
👨🔬 We detect enrichment of EcN in tumor samples over normal tissue from probiotic-treated patients (primary outcome of the trial). Next, we develop early CRC intervention strategies. To detect lesions, we engineer EcN to produce a small molecule, salicylate.
🧫 Oral delivery of this strain results in increased levels of salicylate in the urine of adenoma-bearing mice, in comparison to healthy controls. To assess therapeutic potential, we engineer EcN to locally release a cytokine, GM-CSF, and blocking nanobodies against PD-L1 and CTLA-4 at the neoplastic site, and demonstrate that oral delivery of this strain reduces adenoma burden by ~50%.
👩🔬 Together, these results support the use of EcN as an orally-deliverable platform to detect disease and treat CRC through the production of screening and therapeutic molecules.
🎤 To find out more, meet Susan Woods from University of Adelaide Alumni - School of Medicine, AUSTRALIA at the 17th IPC2024 in Prague, 18-21 June 2024 and
...Hit the bell 🔔🔔🔔 to stay tuned on to International Scientific Conference on hashtag#Probiotics, hashtag#Prebiotics, hashtag#Gut hashtag#Microbiota and hashtag#Health - IPC2024 and get updated on probiotic, prebiotic hashtag#science and hashtag#microbiota hashtag#research
🔬🧫🧬 Bioengineered probiotics enable new opportunities to improve colorectal cancer (CRC) screening, prevention and treatment.
👩⚕️ Here, first, we demonstrate selective colonization of colorectal adenomas after oral delivery of probiotic E. coli Nissle 1917 (EcN) to a genetically-engineered murine model of CRC predisposition and orthotopic models of CRC.
🧬 We next undertake an interventional, double-blind, dual-centre, prospective clinical trial, in which CRC patients take either placebo or EcN for two weeks prior to resection of neoplastic and adjacent normal colorectal tissue (ACTRN12619000210178).
👨🔬 We detect enrichment of EcN in tumor samples over normal tissue from probiotic-treated patients (primary outcome of the trial). Next, we develop early CRC intervention strategies. To detect lesions, we engineer EcN to produce a small molecule, salicylate.
🧫 Oral delivery of this strain results in increased levels of salicylate in the urine of adenoma-bearing mice, in comparison to healthy controls. To assess therapeutic potential, we engineer EcN to locally release a cytokine, GM-CSF, and blocking nanobodies against PD-L1 and CTLA-4 at the neoplastic site, and demonstrate that oral delivery of this strain reduces adenoma burden by ~50%.
👩🔬 Together, these results support the use of EcN as an orally-deliverable platform to detect disease and treat CRC through the production of screening and therapeutic molecules.
🎤 To find out more, meet Susan woods from University of Adelaide School of Medicine, AUSTRALIA at the 17th IPC2024 in Prague, 18-21 June 2024 and
...Hit the bell 🔔🔔🔔 to stay tuned on to International Scientific Conference on #Probiotics, #Prebiotics, #Gut #Microbiota and #Health - IPC2024 and get updated on probiotic, prebiotic #science and #microbiota #research.
🔬🧫🧬 Exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) have gained attention due to their potential health benefits.
👩⚕️ This study focuses on the EPS derived from Enterococcus faecium (EPS-LB13) and Streptococcus thermophilus (EPS-MLB10) isolated from Labneh, a traditional Middle Eastern fermented dairy product and their prebiotics nature.
🧬 These EPSs also supported the growth of beneficial gut bacteria from various phyla, including Proteobacteria, Bacteroidetes, Firmicutes, and Acinetobacter during faecal fermentation. The total short-chain fatty acids (SCFAs) production, a key indicator of gut health, ranged from 5548 to 6023 PPM.
👨🔬 The findings of this study shed light on the promising potential of EPS derived from Enterococcus faecium and Streptococcus thermophilus in promoting human health and well-being. The observed bioactivities, including antioxidant, anticancer, and antimicrobial effects, underscore the functional attributes of these EPS, indicating their potential applications in functional foods and nutraceuticals.
🧫 Furthermore, the positive impact of EPS on the gut microbiome is of significant importance, as it contributes to the modulation of the intestinal environment and the promotion of intestinal homeostasis. The ability of EPS to support the growth of beneficial gut bacteria and promote the production of short-chain fatty acids underscores their potential as prebiotics.
🎤 To find out more, meet Mohammed Tarique at the 17th IPC2024 in Prague, 18-21 June 2024 and...
Hit the bell 🔔🔔🔔 to stay tuned on to International Scientific Conference on #Probiotics, #Prebiotics, #Gut #Microbiota and #Health - IPC2024 and get updated on probiotic, prebiotic #science and #microbiota #research.
#exopolysaccharides
🔬🧫🧬 Cross-talk with the host is one of the most important attribute of probiotic organisms to mediate their functional properties.
🧫 Expediting the transit period of enteric pathogens in the gastrointestinal tract by inhibiting their binding and modulating the immune system of the host are important steps in defense against pathogens.
👩⚕️ The hypothesis of this study was that isolated and purified SlpH from L. helveticus might also interact with the gut lining to compete and exclude enteric pathogens.
🧬 The pathogen exclusion and competition activity and tolerance to harsh gastrointestinal conditions shows promise in SlpH as a potential prophylactic or therapeutic agent against intestinal pathogens.
👨🔬 The secondary structure analysis indicated its stability under low pH justifies that it could be used as nutraceutical.
🎤 To find out more, meet Ritu Patel from Indrashil University at the 17th IPC2024 in Prague, 18-21 June 2024 and
Hit the bell 🔔🔔🔔 to stay tuned on to International Scientific Conference on #Probiotics, #Prebiotics, #Gut #Microbiota and #Health - IPC2024 and get updated on probiotic, prebiotic #science and #microbiota #research.
🔬🧫🧬 The human gastrointestinal tract serves as a dynamic habitat for a vast and intricate community of microbes, engaging in sophisticated interactions with host cells that profoundly influence and contribute to diverse physiological functions.
👩⚕️ A 6-month in vivo investigation involved 35 female C57BL/6J mice allocated into four groups: control diet (receiving water), high-fat diet (with water), high-fat diet with L. paracasei 11w, and high-fat diet with L. paracasei 11w supplemented with 4% GOS in drinking water.
🧬 After a 6-month dietary intervention, there were no statistically significant alterations in body weight. However, both the groups administered with L. paracasei 11w and L. paracasei 11w + GOS exhibited enhanced glucose metabolism compared to the high-fat diet with water group. Moreover, these groups demonstrated beneficial effects on depression- and anxiety-like behaviors, along with improvements in memory function as assessed through behavioral tests.
👨🔬 Collectively, the findings of this investigation underscore the potential of the L. paracasei 11w and L. paracasei 11w + GOS groups as promising psychobiotic and antidiabetic agents. Evident enhancements in glucose metabolism and mental health outcomes were observed.
🧫 Nevertheless, it is imperative to acknowledge the need for further research to delve into the specific role of these probiotic interventions in the context of type 2 diabetes and associated neurological conditions.
🎤 To find out more, meet Ashwinipriyadarshini Megur at the 17th IPC2024 in Prague, 18-21 June 2024 and...
Hit the bell 🔔🔔🔔 to stay tuned on to International Scientific Conference on #Probiotics, #Prebiotics, #Gut #Microbiota and #Health - IPC2024 and get updated on probiotic, prebiotic #science and #microbiota #research.
🔬🧫🧬 The gastrointestinal tract is the main ecological niche in which Lactobacillus strains provide health benefits in mammals. There is currently a need to better characterize host-microbe interactions in space and time by tracking these bacteria.
👩⚕️ First, we engineered different luciferase-expressing probiotic strains and demonstrated that the click-beetle red luciferase is the best performing system for noninvasive in vivo bioluminescence imaging in healthy anesthetized mice.
🧬 Then, we developed fluorescent #Lactobacillus strains and combined noninvasive whole-body imaging with ex vivo fluorescence confocal #microscopy imaging to monitor the impact of intestinal inflammation on the persistence of orally administered Lactobacillus in healthy and inflamed mouse colons.
👨🔬 Finally, we recently started to investigate the interactions of Lactobacilli at the interface of a 3D human intestinal epithelium reconstituted in an organ-on-chip system.
🧫 Overall, our ability for monitoring microbial spatio-temporal dynamics at tissue levels using in vivo and ex vivo models allows us to better characterize host-microbiome interactions in the gut.
🎤 To find out more, meet Catherine Daniel at the 17th IPC2024 in Prague, 18-21 June 2024 and
Hit the bell 🔔🔔🔔 to stay tuned on to International Scientific Conference on #Probiotics, #Prebiotics, #Gut #Microbiota and #Health - IPC2024 and get updated on probiotic, prebiotic #science and #microbiome #research.
🔬🧫🧬 Camel milk has been scientifically demonstrated to possess inherent anti-diabetic properties, a quality that can be further potentiated through the incorporation of lactobacilli.
👩⚕️ The primary focus of this investigation was to evaluate the potential health-promoting attributes of these LAB, including their proteolytic activity, α-glucosidase inhibition, DPP-IV inhibition, and probiotic properties.
🧬 A total of 188 lactic acid bacteria were identified from raw camel milk, with 52 exhibiting proteolytic properties. Among these strains, 16 showed DPP-IV inhibitory activity, and 12 exhibited α-glucosidase inhibitory activities.
👨🔬 Isolated lactic acid bacteria from raw camel milk, displaying proteolytic and curd-forming properties, were characterized for their probiotic potential. Selected strains, including Lacticaseibacillus rhamnosus, exhibited inhibitory activities against DPP-IV and α-glucosidase.
🧫 In a rat model, these strains led to significant reductions in blood glucose levels and showed diverse systemic effects, suggesting potential applications in glycemic control and metabolic health.
🎤 To find out more, meet Mohammad Rizwan at the 17th IPC2024 in Prague, 18-21 June 2024 and
Hit the bell 🔔🔔🔔 to stay tuned on International Conference on Probiotics, Prebiotics, Gut Microbiota & Health - IPC2024 and get updated on probiotic, prebiotic #science and #microbiota #research.
🔬🧫🧬 Antimicrobial resistance (AMR) is undermining modern medicine, a problem compounded by bacterial adaptation to antibiotic pressures.
👩⚕️ Phages are viruses that infect bacteria. Their diversity and evolvability offer the prospect of their use as a therapeutic solution. Reported are outcomes of customized phage therapy for patients with difficult-to-treat antimicrobial resistant infections.
🧬 We retrospectively assessed 12 cases of customized phage therapy from a phage production center.
👨🔬 Fifty requests for phage therapy were received. Customized phages were generated for 12 patients. After treatment, 42% (5/12) of cases showed bacterial eradication and 58% (7/12) showed clinical improvement, with two-thirds of all cases (66%) showing favorable responses.
🧫 Customized phage production and therapy was safe and yielded favorable clinical or microbiological outcomes in two-thirds of cases. A center or pipeline dedicated to tailoring the phages against a patient's specific AMR bacterial infection may be a viable option where standard treatment has failed.
🎤 To find out more, meet Sabrina Green from KU Leuven at the 17th IPC2024 in Prague, 18-21 June 2024 and
Hit the bell 🔔🔔🔔 to stay tuned on to International Scientific Conference on #Probiotics, #Prebiotics, #Gut #Microbiota and #Health - IPC2024 and get updated on probiotic, prebiotic #science and #microbiota #research.
🔬🧫🧬 Beneficial properties that Bacteriocins cultures can bring to the fermented food products will be resulting in reduction of chemical additives and cover exigences of the consumers for more natural and freer of the artificial preservatives food commodities, for the improvement of food safety and in the combating of microbial infections.
👩⚕️ The number of research projects that are focused upon the isolation and characterization of bacteriocins have continued to escalate and according to www.scopus.com, more than 14.474 research papers have been published concerning different aspects of the production, characterization, and application of bacteriocins.
🧬 Research and screening for new strains with more than one beneficial property were accelerated with aim to implement in the food fermentation processes microbial starter that can be considered as multifunctional and if possible, to be with probiotic or bioprotective properties in addition to their starter cultures performance.
👨🔬 Safety has always been and will be driving priority in selection of new functional microbial cultures.
🧫 Bacteriocins can be clearly associated with controlling spoilage and pathogens, extending shelf life, and replacing chemical preservatives. With new knowledge of bacteriocinogenic LAB a more scientific questions will need to be addressed and answers provided.
🎤 To find out more, meet Svetoslav Todorov at the 17th IPC2024 in Prague, 18-21 June 2024 and
Hit the bell 🔔🔔🔔 to stay tuned on to International Scientific Conference on #Probiotics, #Prebiotics, #Gut #Microbiota and #Health - IPC2024 and get updated on probiotic, prebiotic #science and #microbiota #research.
